Iron Maiden were ranked #24 in VH1's "100 Greatest Artists of Hard Rock". The band were ranked fourth on MTV's "Top 10 Greatest Heavy Metal Bands of All Time". Iron Maiden were named as the third best heavy metal band of all time on VH1 Classic: Top 20 Metal Bands. The band also won the Ivor Novello Award for international achievement in 2002. The band was also inducted into the Hollywood RockWalk during their tour in the United States during 2005.

Iron Maiden frequently use the slogan "Up the Irons" in their disc liner notes, and the phrase can also be seen on several t-shirts officially licensed by the band. "The Irons" has been used to refer to the London football club, West Ham United, of which founder Steve Harris is a fan. Fans of Iron Maiden have been known to use the phrase as a greeting or sign-off to other Iron Maiden fans.

Iron Maiden's mascot, Eddie, is a perennial fixture in the band's science fiction and horror-influenced album cover art, as well as in live shows. Eddie was drawn by Derek Riggs until 1992, although there have been various versions by numerous artists including Melvyn Grant. Eddie is also featured in a first-person shooter video game from the band, Ed Hunter, as well as numerous books, graphic comics and band-related merchandise.

In 2008, Kerrang! released an album, entitled Maiden Heaven: A Tribute to Iron Maiden, composed of Iron Maiden cover songs played by artists such as Metallica, Machine Head, Dream Theater, Trivium, Coheed and Cambria, Avenged Sevenfold, and others who were influenced by Iron Maiden throughout their careers. Well over a half-dozen other Iron Maiden tribute albums (each featuring various artists) exist, including a piano tribute, an electro tribute, a black metal tribute and a hip-hop tribute.

Iron Maiden songs have been featured in the soundtracks of several video games, including Carmageddon 2, Grand Theft Auto: Vice City, Grand Theft Auto: Episodes From Liberty City, Grand Theft Auto IV: The Lost and Damned, Tony Hawk's Pro Skater 4, SSX on Tour and Madden NFL 10. Their music also appears in the Guitar Hero and Rock Band series of rhythmic video games. Iron Maiden songs have also appeared on the films Phenomena (called "Creepers" in the U.S.), and Murder by Numbers; while MTV's animated duo Beavis and Butthead have commented favorably on Iron Maiden multiple times.

The band's name is named prominently (and repeated several times) in the songs "Teenage Dirtbag" by Wheatus and "Back to the 80's" by Danish dance-pop band Aqua.

Punk rock band NOFX released a song titled "Eddie, Bruce, and Paul" on their 2009 album Coaster. This track tells the story of Iron Maiden, and is performed in the style of their early work.

Weezer mentions them in the song "Heart Songs" from their 2008 self-titled "Red" album. The verse goes: "Iron Maiden, Judas Priest, and Slayer taught me how to shred..."

The Sum 41 song "Fat Lip" contains the line "Heavy metal and mullets it's how we were raised, Maiden and Priest were the gods that we praised"